|
KMID : 0385019980140020151
|
|
Korean Journal of Laboratory Animal Science
1998 Volume.14 No. 2 p.151 ~ p.155
|
|
|
Intestinal Morphology and Brush Border Membrane D-Glucose Transport from SHR Rats Treated with Streptozotocin
|
|
김용규/Kim, Yong Kyu
조정식/채갑용/임철주/강태석/장인석/김철규/Cho, Jung Sik/Chae, Kab Ryong/Lim, Chul Joo/Kang, Tae Seok/Jang, In Surk/Kim, Chuel Kyu
|
|
|
Abstract
|
|
|
|
The current study was designed to determine the effect of diabetes mellitus induced by streptozotocin on intestinal function as indicated by changing intestinal morphology and Na^+-dependent glucose transport across intestinal brush border membrane vesicles (BBMV) from SHR rats. The SHR rats aged 10.5wk were treated with streptozotocin (STZ, 70㎎/㎏ i.p) to induce diabetes mellitus. The treatment of STZ gave rise to decrease in body weight and increases in blood glucose, triglyceride, and cholesterol levels except insulin at death. In addition, enhanced intestinal length, mucosal mass, and mucosal sucrase specific activity were observed in STZ group compared with those in control group. Electronmicroscopic examination of the BBM in the duodenum from SHR rats revealed that microvilli height and density were basically identical in both control and STZ groups. To measure intestinal D-glucose transport, we isolated BBM and identified purity of the intestinal BBM. The average enrichments of intestinal alkaline phosphatase, sucrase, and Na^+/K^+-ATPase were over 12.3, 14.0 and 2.9 fold, respectively, suggesting that our isolation procedure yielded highly purified BBM. In glucose transport expressed as ㎎ protein per BBMV (nM/㎎ protein/sec), the Na^+-dependent glucose transport was not influenced by treatment of STZ. The apparent K_m and V_(max) values for active Na^+-dependent uptake of glucose were similar between two groups. However, when comparing Na^+-dependent glucose uptake expressed as total protein per BBMV (nM/BMV/sec), STZ treatment significantly increased glucose uptake because of intestinal hyperplasia.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|